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Table 2 Primers for overlapping PCR

From: Experimental immunization of mice with a recombinant bovine enterovirus vaccine expressing BVDV E0 protein elicits a long-lasting serologic response

Primer name

Primer sequence (5′ to 3′)

Product size (bp)

P23-F

GCAATAGGCGTTATAACATTGGGAACGTCCTCGAGGCACTCTTCCAGGGCGGCCGCTACCCATACGAC

1317

P23-3CF

GGCGGCCGCTACCCATACGACGTCCCAGACTACGCTTCTAGAGCACTCTTCCAGGGCCCAGTTTGCTACAAACCCCTC

1270

P3-R

CCAGGGCCTCCAAGCCTTCAGTAC

 
  1. Note:NotI (GCGGCCGC) and XbaI (TCTAGA) enzyme double restriction sites are italic, bold and underlined
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Virology Journal

ISSN: 1743-422X