Fifteen new nucleotide substitutions in variants of human papillomavirus 18 in Korea

Table 1 Primers used for PCR amplification and sequence analysis

Gene	Primer	Trial	Primer sequence (5′-3′)	Position	Size	Success rate	Ref
E6	E6-a-F	1	AGAAACACACCACAATACTATGGCG	86	661	86/170	[21]
	E6-a-R	1	GTCGGGCTGGTAAATGTTGAT	746	661	86/170	[21]
	E6-b-F	2	GGGACCGAAAACGGTGTAT	55	611	11/79	[22]
	E6-b-R	2	GAAGGTCAACCGGAATTTCA	665	611	11/79	[22]
	E6-c-F	3	GGGACCGAAAACGGTGTAT	55	557	0/68	[22]
	E6-c-R	3	ATGTTGCCTTAGGCTCCATGC	611	557	0/68	[22]
E7	E7-a-F	1	CGACAGGAACGACTCCAACGA	540	431	165/170	[21]
E7	E7-a-R	1	ATAAAACCAGCCGTTACAACCCGTG	970	431	165/170	[21]
L1	L1–1-a-F	1	GTAACGGTCCCTTTAACCTCCTC	5402	650	153/170	[21]
	L1–1-a-R		CATTGTCCCTAACGTCCTCAG	6051	650	153/170
	L1–2-a-F		AAGTTCCCATGCCGCCACGTCTAAT	6002	505	162/170
	L1–2-a-R		AGAGCCACTTGGAGAGGGAGAATAC	6506	505	162/170
	L1–3-a-F		GCTCTATTGTTACCTCTGACTCC	6502	636	32/170
	L1–3-a-R		ATTACTTCCTGGCACGTACACGCAC	7137	636	32/170
	L1–3-b-F	2	AGTTATGTATTTTGGGCTGTG	6077	756	0/128	[10]
	L1–3-b-R		ACACCAAAGTTCCAATCCTCTAA	6832	756	0/128
	L1–3-b’-F		AGTATAGCAGACATGTTGAGGAA	6699	551	0/128
	L1–3-b’-R		CATACAACATACAACAACAACCAT	7249	551	0/128
	L1–3-c-F	3	TCCCTCTCCAAGTGGCTCTA	6488	601	44/128	[22]
	L1–3-c-R	3	AGTGGCAGATGGAGCAGAAC	7088	601	44/128	[22]

ISSN: 1743-422X