Fig. 3From: The tyrosine 73 and serine 83 dephosphorylation of H1N1 swine influenza virus NS1 protein attenuates virus replication and induces high levels of beta interferonNS1 protein localization is not affected by Y73F or S83A dephosphorylation. MDCK cells were infected with rSIV and mutated viruses at an MOI of 1. At 6 hpi, the cells were fixed and stained with a specific anti-NS1 polyclonal antibody and a Alexa Fluor 488–labeled secondary antibody (green). Nuclei were stained with DAPI (blue). The localizations of NS1 proteins were visualized using a Carl Zeiss fluorescence microscope. Merge: merged image of double immunofluorescence staining of the same fieldBack to article page