Fig. 5
Mutations of residues M260A and L261A partly restrained C protein degradation. a Schematic representation of mutant C proteins, CM260-261 and CM260-263, in which residues 260-261 and 260-263 in C protein are all mutated to Alanine (A), respectively. Mutant residues are shown above the oblong. b CM260-261 and CM260-263 were expressed in PK-15 cells followed by treatment of MG132 or DMSO as described above. Western blot was performed and CM260-261 and CM260-263 were detected with the indicated antibodies. Protein marker is shown on the right