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Table 6 Virus titer determination in samples prepared with different combination of Sabin strains and analyzed in blind format with MPBT assay

From: Multiplex PCR-based titration (MPBT) assay for determination of infectious titers of the three Sabin strains of live poliovirus vaccine

Samples codes

Spiked CCID50 titera (Sabin type)

Results of MPBT assay

Sabin type

Titera

A

7.63 (1)

1

7.5

B

7.04 (2)

2

7

C

7.42 (3)

3

7.75

D

7.63 (1)

1

7.38

 

7.04 (2)

2

6.75

E

7.63 (1)

1

7.38

 

7.42 (3)

3

7.5

F

7.04 (2)

2

6.88

 

7.42 (3)

3

7.25

G

7.63 (1)

1

7.25

 

7.04 (2)

2

7.25

 

7.42 (3)

3

7.75

  1. aTiters of Sabin strains are expressed on log10CCID50/ml