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Table 1 Titration of different lots of Sabin strains with both MPBT and CCID50 assays

From: Multiplex PCR-based titration (MPBT) assay for determination of infectious titers of the three Sabin strains of live poliovirus vaccine

Sabin type (lot)

Single titrationa

Multiplex titrationa

Mean

SD

RSD (%)

MPBT assay

CCID50 assay

MPBT assay

1 (1)

8.75

8.75

9.13

8.88

0.22

53.92

2 (1)

8.63

8.63

8.63

8.63

0.00

0.00

3 (1)

9.36

9.25

9

9.20

0.18

44.47

1 (2)

8.6

8.63

8.62

8.62

0.02

3.52

2 (2)

8.19

8.04

8.18

8.14

0.08

19.49

3 (2)

8.57

8.42

8.49

8.49

0.08

17.41

1 (3)

8.52

8.43

8.56

8.50

0.07

15.42

2 (3)

8.31

7.97

8.26

8.18

0.18

44.23

3 (3)

8.43

8.39

8.34

8.39

0.05

10.41

1 (4)

7.77

7.77

8.07

7.87

0.17

41.52

2 (4)

7.73

7.65

8.14

7.84

0.26

66.52

3 (4)

8.1

7.94

7.84

7.96

0.13

30.90

  1. aThe titers are in Log10CCID50/ml, RSD Relative standard deviation, SD Standard deviation of log10 titers, Single titration; Each Sabin Strain was titrated separately, Multiplex titration; All 3 Sabin strains were titrated in the same reaction