Fig. 7From: Identification of virion-associated transcriptional transactivator (VATT) of SGIV ICP46 promoter and their binding site on promoterEffect on SGIV ICP46 promoter activity by transient expression of four VATTs. (a) Expression of four VATTs in GS cells verified by RT-PCR after transfected with pcDNA-012, pcDNA-039, pcDNA-057 and pcDNA-072 at 24 h post tansfection . lanes M, 10 kb DNA ladder; Lanes C, negtive control. (b) The luciferase reporter assays after co-transfection with pcDNA-012, pcDNA-039, pcDNA-057, pcDNA-072 alone or 4 plasmids combined. At 24 h post transfection, cells were infected with SGIV and harvested at 6 h p.i.. The co-transfected cells without SGIV infection were harvested at the same time for luciferase reporter assays. Firefly luciferase activities were normalized based on the activity of Renilla luciferase. The mean Fluc/Rluc intensity of negative control was considered as 1Back to article page