Fig. 2

miR-27b-mediated suppression of AQP11 expression in Huh7.5.1 cells. a AQP11 mRNA levels in Huh7.5.1 cells following transfection with a miR-27b mimic. Huh7.5.1 cells were transfected with 25 nM of miR-27b mimic or control mimic. Following a 48-h incubation, total RNA was recovered from the cells, followed by real-time RT-PCR analysis. AQP11 mRNA levels were normalized to GAPDH. b AQP11 protein levels in Huh7.5.1 cells following transfection with a miR-27b mimic. Huh7.5.1 cells were transfected with 50 nM of miR-27b mimic or control mimic. Following a 72-h incubation, cell lysates were prepared, followed by western blot analysis. Representative image from two independent experiments are shown. c AQP11 mRNA levels in Huh7.5.1 cells following transfection with antagomiR-27b. Huh7.5.1 cells were transfected with 10 nM of antagomiR-27b or control antagomir. Following a 48-h incubation, total RNA was recovered, followed by real-time RT-PCR analysis. AQP11 mRNA levels were normalized to GAPDH. D, relative renilla luciferase activities following co-transfection with a reporter plasmid containing the 3′-UTR sequence of the AQP11 gene and miR-27b mimic. HEK293 cells were transfected with 25 nM of miR-27b mimic or control mimic and 1 μg/ml of reporter plasmids. Following a 72-h incubation, renilla and firefly luciferase activities were determined. Renilla luciferase activity levels were normalized to firefly luciferase activity. The data are expressed as the means ± S.D. (N = 3). *p < 0.05