Table 6 Evaluation of the clinical performance of the multiplex RT-qPCR assay for the detection and genotyping of equine rotavirus A in fecal samples compared to VP7-specific RT-PCR and sequencing (gold standard). (a) NSP3 (b) G3 VP7 and (c) G14 VP7
a | ||||
VP7-specific RT-PCR | ||||
Positive | Negative | Total | ||
NSP3-specific RT-qPCR | Positive | 85 | 0 | 85 |
Negative | 0 | 92 | 92 | |
Total | 85 | 92 | 177 | |
b | ||||
ERVA genotype G31 | ||||
Positive | Negative | Total | ||
G3-specific RT-qPCR | Positive | 38 | 0 | 38 |
Negative | 3 | 136 | 139 | |
Total | 41 | 136 | 177 | |
c | ||||
ERVA genotype G141 | ||||
Positive | Negative | Total | ||
G14-specific RT-qPCR | Positive | 44 | 1 | 45 |
Negative | 0 | 132 | 132 | |
Total | 44 | 133 | 177 | |