Fig. 1From: Bovine leukemia virus long terminal repeat variability: identification of single nucleotide polymorphisms in regulatory sequencesScheme of the overlap extension PCR (OE-PCR) procedure. First, two overlapped LTR fragments are generated by regular PCR. Second, a DNA multimer is formed by OE-PCR without primers and with a prolonged extension time. The method significantly facilitate precise in-frame assembly of two LTR fragmentsBack to article page