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Fig. 5 | Virology Journal

Fig. 5

From: Ubiquitin-conjugating enzyme UBE2J1 negatively modulates interferon pathway and promotes RNA virus infection

Fig. 5

UBE2J1 mainly promotes K48 -linked ubiquitination of IRF3. a HEK293T cells were transfected with plasmids of UBE2J1 shRNA (or N.C.) and then infected with DENV for 24 h. RIG-I, MAVS, TRAF3, TBK1, IKKε, and IRF3 protein levels were detected by immunoprecipitation with indicated primary antibodies. The increased IRF3 protein level was indicated by stars (**). (b-d) UBE2J1 interacted with IRF3 and facilitated IRF3 ubiquitination. HEK293T cells were transfected with Flag-IRF3, HA-Ub (b), (or HA-K48-Ub (c)/HA-K63-Ub (d)), along with UBE2J1-His or control plasmids for 24 h. Cells were then treated with MG132 for 6 h before harvesting the cells lysates. Distinct types of ubiquitinated IRF3 proteins were detected by immunoprecipitation and western blotting with indicated tag antibodies

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Virology Journal

ISSN: 1743-422X