Experimental infection of mice with noncytopathic bovine viral diarrhea virus 2 increases the number of megakaryocytes in bone marrow

Table 2 BVDV detection by IHC in mice inoculated orally with a low or high dose of ncp BVDV-2

Day	Day 2								Day 5								Day 9
Day	L1	L2	L3	L4	H1	H2	H3	H4	L1	L2	L3	L4	H1	H2	H3	H4	L1	L2	L3	L4	H1	H2	H3	H4
Tissues	L1	L2	L3	L4	H1	H2	H3	H4	L1	L2	L3	L4	H1	H2	H3	H4	L1	L2	L3	L4	H1	H2	H3	H4
BALT	−	−	−	−	−	−	−	−	−	−	−	−	−	−	−	+	−	−	−	−	−	−	−	−
Bone marrow	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
Mesenteric lymph node	−	−	+	+	+	−	−	−	−	−	+	+	−	−	−	+	−	−	−	−	−	−	−	−
Peyer’s patches	−	−	−	+	+	−	−	−	−	−	−	−	−	−	−	+	−	−	−	−	−	−	−	−
Spleen	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+

Eight mice were euthanized at each time point. Four mice were inoculated with a low dose, and four were inoculated with a high dose of the virus. Three independent experiments were conducted and the results were the same. The data are representative of three independent experiments
L: low dose (4 × 10⁵ TCID₅₀); H: high lose (1.2 × 10⁶ TCID₅₀)
“−”: not detected, “+”: detected
BALT: bronchus-associated lymphoid tissue

ISSN: 1743-422X