Fig. 1
From: Evaluation of a method to measure HHV-6B infection in vitro based on cell size
Cytopathic effect in HHV-6B-infected SupT1. a. Phase-contrast microscopy images (20×) of SupT1 cells suspension non-infected and infected at different days post-infection (dpi); bar = 100 μm. b. Flow cytometry scatter plots (FSA vs SSA) of non-infected and infected SupT1 cells at 4 and 7 dpi. C. SEM images (2,000×) of non-infected and infected SupT1 at 4 dpi. Bar: 30 μm. d. TEM images (43,000×) of infected SupT1, showing different stages of virus replication: nucleocapsids in the nucleus (white arrowheads), tegumented virus acquiring envelope in the cytoplasm (black arrowheads), and virions in the extracellular space (arrows); bars = 0.2 μm. e. Negative staining (20,500×) of virus purified from supernatant of infected cells; arrows show virions; bar = 0.5 μm. f. Viral gene expression in SupT1 non-infected or infected with live or inactivated virus (HI = heat-inactivated; UVI = UV-inactivated) at 4 and 7 dpi. Agarose gel shows PCR products for U86 (top), U12 (middle) and beta-actin (bottom) transcripts for each condition