The nonstructural proteins of Pneumoviruses are remarkably distinct in substrate diversity and specificity

Table 1 Contrasting activity of RSV and PVM nonstructural proteins on select ISGs

ISG name	GenBank#	% Reduction by RSV NS1 or NS2	% Reduction by PVM NS1 or NS2 [24]
IFIT1	NM_001548	84% NS1, 17% NS2	Not done
IFITM3	NM_021034	82% NS1, 17% NS2	Not done
MAPK8	AB451231.1	2% NS1, 33% NS2	Not done
IFITM1	NM_003641	7% NS1, 5% NS2	63% NS1, 32% NS2
TRAFD1	NM_006700	16% NS1, 0% NS2	68% NS1, 76% NS2
ISG20	NM_002201	23% NS1, 0% NS2	18% NS1, 54% NS2

This is a numerical summary of reduction of the selected ISGs by the two pneumoviral NS proteins, expressed individually. The RSV data were calculated with the average band intensities of the immunoblot of Fig. 1a, which were also graphically plotted in Fig. 1b, and the PVM data were calculated by densitometry of the corresponding immunoblot in our recent publication [24]. “Not done” indicates ISGs for which recombinant mouse homologs were unavailable. Note that in this Table we present the percent reduction of the ISG, whereas in Fig. 1b (bar graph), the percent remaining is plotted; thus 84% reduction of ISG here corresponds to 16% remaining ISG in Fig. 1b. RSV NS proteins affected the upper three ISGs are more strongly than the lower three

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