Fig. 2

Agrobacterium pTRA plant expression vectors: pTRAc (a), pTRAkc-ERH (b), pTRAkc-rbcs1-cTP (c) and pRIC 3.0 (d). LB and RB, left and right borders for T-DNA integration; SAR, scaffold attachment region of the tobacco Rb7 gene; P35SS, CaMV 35S promoter with duplicated transcriptional enhancer; CHS, chalcone synthase 5′ untranslated region; pA35S, CaMV 35S polyadenylation signal; RK2 ori, origin of replication for A. tumefaciens; bla, ampicillin/carbenicillin-resistance bla gene; ColE1 ori, origin of replication for E. coli; LPH, signal peptide sequence from the murine mAb24 heavy chain; 6xHis, 6 His tag sequence; SEKDEL, ER-retention signal sequence; rbcs1-cTP, chloroplast-transit peptide sequence of a Rubisco small-subunit gene (rbcS1) from S. tuberosum; npt II, kanamycin-resistance npt II gene; Pnos and pAnos, promoter and polyadenylation signal of the nopaline synthase gene; LIR, BeYDV long intergenic region; rep/repA, BeYDV rep/repA gene; SIR, BeYDV short intergenic region