Fig. 1
From: Molecular characterization of the duck enteritis virus US10 protein
Expression, identification, and purification of the recombinant US10 protein. a Expression of the recombinant protein. Total protein stain. Lane M: markers; lanes 1–3: the whole bacterial lysate, supernatant, and inclusion bodies of pET32a(+)/US10; lanes 4 and 5: the induced and uninduced pET32a(+). b Identification of the recombinant protein by western blotting with anti-DEV serum; lane M: markers; lane 1: pET32a(+); lanes 2–4: the whole bacterial lysate, supernatant, and inclusion bodies of pET32a(+)/US10. c Purification of the recombinant US10 protein. Detection with anti-DEV serum. Lane M: markers; lane 1: purified recombinant pET32a(+)/US10 protein