Fig. 4

Inhibitor kinetics experiments. HEp-2 cells were treated with AEBSF, infected with RSV A2 for 2 h and incubated at 37 °C for an additional 16 h. Cells were fixed, permeabilized, incubated with a pAb goat anti-RSV antibody and stained with AF488 donkey anti-goat (IgG). Nuclei were visualized with DAPI and analyzed with fluorescence microscopy. The percentage of RSV positive cells was calculated as the number of positive cells relative to the total number of nuclei. a HEp-2 cells were treated with 0.3 mM AEBSF pre-, peri- or post-inoculation or combinations of these three as indicated below the graph. Data represents means (±SEM) of 3 independent repeats, significant differences compared to the untreated infection control (IC) are indicated by *p < 0.05, **p < 0.01, ***p < 0.001. b HEp-2 cells were treated with 0.3 mM AEBSF peri-, post- or a combined treatment of peri- and post-inoculation. Cells were incubated with virus for 2 h at 4 °C to allow attachment but not fusion. Cells were shifted to 37 °C for an additional 16 h. Results are expressed as means (±SEM) (n = 3), significant differences compared to the untreated infection control (IC) are indicated by *p < 0.05, **p < 0.01, ***p < 0.001