Development and application of triple antibody sandwich enzyme-linked immunosorbent assays for begomovirus detection using monoclonal antibodies against Tomato yellow leaf curl Thailand virus

Table 2 Detection and identification of begomoviruses in field samples by TAS-ELISA, PCR and DNA sequence analysis

Host	No. of samples tested	No. of samples positive by PCR	TAS-ELISA^e		Species based on sequence analysis of cp gene
Host	No. of samples tested	No. of samples positive by PCR	No. of samples positive by M1	No. of samples positive by D2	TYLCTHV	TbLCYnV	ToLCNDV	SLCCNV	PepLCV	AYVV	BYVMV	PepYLCIV	TYLCKaV
Tomato	37	27	15	21	11^a	4^a	1^b			9^(5c,4d)			2^d
Cucumber	39	29	0	29			29^b
Loofah	6	1	0	1			1^b
Ivy gourd	1	1	0	1			1^b
Melon	9	4	0	4			2^b	2^b
Pumpkin	5	5	0	5				5^b
Wax gourd	2	2	0	2				2^b
Eggplant	5	5	0	0									5^d
Okra	5	5	0	0							5^d
Pepper	34	34	0	9					24^(9c,15d)			8^d	2^d
Weed	4	4	1	1		1^a			1^d	2^d
Total	147	117	15	73	11	5	34	9	25	11	5	8	9

ISSN: 1743-422X