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Table 6 Validation of plasmids containing the HBV DNA sequences of samples with higher viral loads (at least 1.0 log10IU/ml) detected with the Roche kit was 1.0 log10IU/ml lower than that of duplex real-time PCR assay

From: Real-time PCR assays for hepatitis B virus DNA quantification may require two different targets

Sample No.

Concentrationa

Roche

Daan

Duplex-S

Duplex-C

Wild

1.0e4

1.79e4

1.01e4

1.7e4

1.5e4

302-44b

1.0e4

3.09e2

7.41e3

No

No

302-45

1.0e4

5.29e3

5.16e3

6.19e3

9.03e3

302-66

1.0e4

2.45e3

3.83e3

1.42e4

1.0e4

302-76b

1.0e4

2.36e2

5.37e3

1.27e4

1.49e4

  1. aCalculated from the concentration of the plasmid
  2. bThe samples for which quantitative results by Roche were more than 1.0log10IU/ml than that of other assays (Unit: IU/ml)
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Virology Journal

ISSN: 1743-422X