Figure 4From: Functional exchangeability of the nuclear localization signal (NLS) of capsid protein between PCV1 and PCV2 in vitro: Implications for the role of NLS in viral replicationOne-step growth curve of PCV1, PCV2, PCV1-NLS2 and PCV2-NLS1 viruses. Synchronized PK-15 cells were infected with viruses all at an MOI of 0.1. The infectious titers for all viruses were similarly lower than 101.8 TCID50/ml after initial infection, but gradually increased from 12 to 96 hours. PCV1 propagated much more efficiently in vitro with a significantly higher titer of 104.3 TCID50/ml by 96 h post-infection. The infectious titers of viruses were determined by IFA with Reed-Muench method.Back to article page