Thermal stability and inactivation of hepatitis C virus grown in cell culture

Table 3 Effects of detergent treatments on HCVcc infectivity

Treatment	Infectious titers before treatment (FFU/ml)^a	FFU assay after treatment	IFA result at the 3^rd cell passage
0.1% SDS
HCVcc in culture medium	(4.1 ± 0.2) × 10⁴	negative	negative
HCVcc in human serum	(9.5 ± 0.7) × 10⁴	negative	negative
cell lysates	(5.5 ± 0.1) × 10⁵	negative	negative
0.2% Triton X-100
HCVcc in culture medium	(2.0 ± 0.2) × 10⁴	negative	negative
HCVcc in human serum	(6.0 ± 0.0) × 10⁴	negative	negative
cell lysate	(3.1 ± 0.2) × 10⁵	negative	negative
0.2% NP-40
HCVcc in culture medium	(1.6 ± 0.2) × 10⁴	negative	negative
HCVcc in human serum	(5.5 ± 0.2) × 10⁴	negative	negative
cell lysate	(2.9 ± 0.2) × 10⁵	negative	negative

^a Untreated viral samples or intracellular viral infectivity in untreated, HCVcc-infected cell lysates prepared by freeze-and-thaw were retitrated in the presence of 0.001% SDS, 0.0005% Triton X-100 or 0.0005% NP-40, respectively, to keep in line with the final concentration of the detergents in the treated samples during titration.

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