Figure 2
![Figure 2](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2F1743-422X-7-274/MediaObjects/12985_2010_Article_1018_Fig2_HTML.jpg)
The expression of GST-ORF2-E protein was analyzed by SDS-PAGE (A) and Western-blotting (B) with an anti-GST monoclonal antibody. Lane 1, BL21 cell lysate before induction of IPTG; Lane 2, BL21 cell lysate after induction of IPTG; Lane 3, Supernatant of cell lysate after sonication and centrifugation; Lane 4, Pellet of cell lysate after sonication and centrifugation, There was a clear band of 29 kDa (arrow) after induction. The protein marker includes 8 bands at 175, 83, 62, 47.5, 32.5, 25, 16.5, and 6.5 kDa.