Figure 2

Kinetics of expression and immunolocalization of the DEV pUL38 in infected DEF cells. A Western blot of lysates from mock-infected or DEV-infected DEF cells with polyclonal antibodies specific to pUL38 protein, showing that pUL38 is expressed as a 51 kDa protein from 8 h onward following infection. B Immunofluorescence detection of pUL38 in mock-infected (a) or DEV-infected DEF cells at 8(c), 18(d), 30(e) and 56 h(f) post-infection. Cells were incubated with preimmune serum(b) pUL38-specific antibody and subsequently stained with fluorescein isothiocyanate (FITC)-conjugated secondary antibody. Nuclei were counterstained with DAPI (blue).