Figure 5

Phenoloxidase activity in haemolymph of infected S. frugiperda larvae. The haemolymph was collected at 72 h p.i., the cell removed by centrifugation and phenoloxidase activity was determined spectrophotometrically using the cell-free hemolymph (113 μg) and L-3, 4-dihydroxyphenylalanine (L-DOPA) as a substrate. The experiment was repeated 3 times. Note that haemolymph from insects infected with the recombinant vSynScathL and vSynKerat showed increased activation of the enzyme.