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Figure 1 | Virology Journal

Figure 1

From: N-terminal basic amino acid residues of Beet black scorch virus capsid protein play a critical role in virion assembly and systemic movement

Figure 1

Replication of BBSV and its mutants in N. benthamiana protoplasts. (A) Schematic representation of BBSV and the six mutants containing changes within the N-terminus of CP. P23 and its translational readthrough product, P82, are needed for viral replication. P7a, P7b and P5’, three small proteins encoded by the middle portion of BBSV genome, are required for viral cell-to-cell movement. P24 encoded by the 3’ portion of the genome is the viral coat protein (CP). Amino acid residues changed in the CP mutants (BM1-5, BM7) are highlighted in red. (B) Northern blot analysis of viral RNAs accumulated in N. benthamiana protoplasts. Bands corresponding to viral genomic (g) RNA and subgenomic (sg) RNAs (sgRNA1 and 2) are indicated on the right. (C) Western blot detection of WT as well as mutant BBSV CPs in transfected protoplasts.

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Virology Journal

ISSN: 1743-422X