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Fig. 5 | Virology Journal

Fig. 5

From: Identification and characterization of lncRNA AP000253 in occult hepatitis B virus infection

Fig. 5

lncRNA AP000253 promoted HBV transcription and replication. (A) The relative level of AP00253 was determined by microarray in liver tissues of CHB (n = 59) and healthy controls (n = 6) from the GSE83148 dataset; (B) The level of AP00253 was detected by qRT-PCR in HepG2, HepG2.2.15, Huh7 and Huh7/pHBV1.3 cells; (C) Validation of overexpression and knockdown efficiency of AP000253 in both HepG2.2.15 and Huh7/pHBV1.3 cells by qRT-PCR, respectively; (D) The levels of HBV DNA, HBsAg and HBeAg were measured by qPCR or ELISA assays in the supernatant of HepG2.2.15 cells treated with si-NC or si-lncRNA AP000253 and pcDNA3.1 or pcDNA3.1/lncRNA AP000253 for 3 days, respectively; (E) The levels of HBV DNA, HBsAg and HBeAg were measured by qPCR or ELISA assays in the supernatant of Huh7/pHBV1.3 cells treated with si-NC or si-lncRNA AP000253 and pcDNA3.1 or pcDNA3.1/lncRNA AP000253 for 3 days, respectively; CHB chronic hepatitis B, HCs healthy controls; ***P < 0.001; **P < 0.01; *P < 0.05, n.s. not significant

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