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Fig. 1 | Virology Journal

Fig. 1

From: Simple rapid in vitro screening method for SARS-CoV-2 anti-virals that identifies potential cytomorbidity-associated false positives

Fig. 1

Drug cytotoxicity, cytomorbidity and inhibition of SARS-CoV-2-induced CPE. The indicated drugs at the indicated concentrations (x axis numbers µg/ml in black, µM in purple) were cultured with Vero E6 cells (i) without virus and 104 cells per well to measure cytotoxicity or MTS activity (black circles and white squares), (ii) without virus and 400 cells per well to measure cytomorbidity (green squares) or (iii) with virus and 104 cells per well to measure viral CPE (red triangles). Crystal violet staining was dissolved in 100% methanol and read at OD595. The mean percentage crystal violet staining or MTS activity (OD490) relative to no drug controls are shown. Error bars represent standard error of the mean (SEM) for 3–6 replicates, with each experiment undertaken independently in triplicate 1–2 times

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