Fig. 1From: A cassava protoplast system for screening genes associated with the response to South African cassava mosaic virusCassava protoplast isolation from leaf mesophyll cells by 16 h-long enzymatic digestion. a M. esculenta 4-week old donor plants cultured on ½ Murashige and Skoog medium. b Protoplasts from model M. esculenta cv.60444 c Protoplasts from susceptible M. esculenta T200 d Protoplasts from tolerant M. esculenta TME3. Spherical protoplasts with chloroplasts around the edge of the cell membrane and central vacuole were observed (shown by red arrows). Protoplasts were visualised under bright field microscopyBack to article page