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Fig. 5 | Virology Journal

Fig. 5

From: Barley yellow dwarf virus-GAV-derived vsiRNAs are involved in the production of wheat leaf yellowing symptoms by targeting chlorophyll synthase

Fig. 5

Silencing of TaChlG by BSMV. a The phenotype in the fourth leaves of healthy plants, BYDV-GAV inoculated plants, BSMV empty vector inoculated plants, PDS-silenced plants, and TaChlG-silenced plants. Photographs were taken at 10 d postinoculation (dpi). b Relative expression levels of PDS and TaChlG in PDS-silenced and TaChlG-silenced plants, respectively. The BSMV empty vector inoculated plants were used as the control. Each relative expression level is presented from three biological samples, and each biological sample had three technical replicates. Error bars represent standard errors of replicates c Relative expression levels of BYDV-GAV in TaChlG-silenced plants. The CP of BYDV-GAV was used to determine the accumulation of virus. TaEF acted as an internal control for normalization in RT-qPCR

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