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Fig. 3 | Virology Journal

Fig. 3

From: Mitomycin, 5-fluorouracil, leflunomide, and mycophenolic acid directly promote hepatitis B virus replication and expression in vitro

Fig. 3

Expression of HBV genes in HBV-NLuc-35 cells is affected by epirubicin, mitomycin, 5-fluorouracil, dexamethasone, leflunomide, and mycophenolic acid. HBV-NLuc-35 cells were exposed to increasing concentrations of cytotoxic chemotherapy drugs, epirubicin (a), mitomycin (b), and 5-fluorouracil (c), for 16 h, and increasing concentrations of immunosuppressants, dexamethasone (d), leflunomide (e), and mycophenolic acid (f), for 5 days. Intact intracellular capsids were detected by immunoblotting with an anti-HBV core protein antibody after NAGE (upper). Luciferase activity in supernatant was determined by the Nano-Glo™ luciferase assay reagent (bottom). Values are the mean of three isodose treatments. Error bars indicate SD. Differences among each concentration are statistically significant (p < 0.05)

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