Fig. 3
From: Identification of emerging viral genomes in transcriptomic datasets of alfalfa (Medicago sativa L.)
a, 3′ RACE and 5′ RACE PCR products generated from the MsAV1-infected tissues. Lane 1: 3′ RACE product amplified with a virus-specific primer GSP 2 and a 3′-CDS primer from the SMARTer RACE kit. Lanes 3,4, and 5: 5′RACE products amplified with different virus-specific primers (GSP1–1, GSP1–2 and GSP1–3, respectively) and a 5′-CDS primer from the SMARTer RACE kit; M: 1 kb Plus DNA Ladder (ThermoFisher Scientific, MA, USA). b, RT-PCR results obtained using primers MsAV1 F1 and MsAV1 R1 from selected alfalfa samples that had a low number of the MsAV1 reads. The arrow indicates RT-PCR amplicons of the expected size (496 bp)