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Table 2 DAS-ELISA reactions of various antisera raised against different plant nucleorhabdoviruses and limited host range study

From: Characterisation of a novel nucleorhabdovirus infecting alfalfa (Medicago sativa)

Host species

Inoculated virus

Antisera

AaNV (JKI-1607)

EMDV (JKI-1073)

PhCMoV (JKI-2051)

N. benthamiana

AaNV

+++a

–

–

P. sativum

AaNV

+

NT b

NT

V. faba

AaNV

+

NT

NT

M. sativa

AaNV

–

NT

NT

M. lupulina

AaNV

–

NT

NT

T. repens

AaNV

–

NT

NT

T. pratense

AaNV

–

NT

NT

C. quinoa

AaNV

–

NT

NT

N. benthamiana

EMDV

–

+++

–

N. benthamiana

PhCMoV

–

–

+++

Buffer

 

–

–

–

N. benthamiana

Non-inoculated

–

–

–

P. sativum

Non-inoculated

–

NT

NT

V. faba

Non-inoculated

–

NT

NT

M. sativa

Non-inoculated

–

NT

NT

  1. aFollowing a substrate incubation for 1 h, DAS-ELISA reactions were classed as follows: negative reaction (−): ≤ cut-off point (= ODA405 0.025); weak reaction (+): cut-off point to 1.0, intermediate reaction (++): 1.0 to 2.0, strong reaction (+++): > 2.0). bNT = not tested
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Virology Journal

ISSN: 1743-422X