Fig. 2
From: Functional analysis of apple stem pitting virus coat protein variants
Analysis of recombinant CPs from different ASPV isolates by SDS-PAGE and western blot. a SDS-PAGE analyze of rCPs expressed in E. coli. Lane M: Protein Ladder; Lanes 1–6 and Lane EV (from left to right): protein extracts from E. coli transformed with vectors pET-HB-HN9–3, pET-HB-HN6–8, pET-LN-AP1–1, pET-HB-HN7–18, pET-HB-HN1–3, pET-YN-MRS-17 and the empty vector pET-28a (+), respectively. b-d Western blot analysis of rCPs by antibody PAb-HB-HN6–8, PAb-YN-MRS-17 and PAb-HB-HN9–3, respectively. Hybridization signals on western blots were quantified by Image J software (right column of B-D)