- aThe extreme 5′ end sequences of three nt variants were identified by sequencing cloned DNA corresponding to the 5′ end of the T30-CA genome. The consensus (black colored) nts located at the 5′ ends of other T30 sequences (GenBank accession nos. KC517489, KC517490, KC517491 and AF260651) were identified in the population of T30-CA in addition to nt variants with an extra nt (“C”, in red). The number of clones for each nt variant sequenced are as indicated
- bThe 5′ end nts of the T30-CA genome was determined by sequencing the (+)-DNA generated by 5′ RLM-RACE using the (+)-strand of dsRNA as a genetic template for reverse transcription (RT) and PCR amplification
- cThe 5′ end nts of the T30-CA genome was determined by sequencing the (−)-DNA generated by 3′ RACE using the (−)-strand of dsRNA as a genetic template for reverse transcription (RT) and PCR amplification
- dThe extreme 3′ end sequences of three nt variants were identified by sequencing cloned (+)-DNA (generated by 3′ RACE) corresponding to the 3′ end of the T30-CA genome. The conserved consensus nts and the variant nts are indicated in black and red, respectively. The number of clones for each nt variant sequenced are as indicated