Figure 4From: Thermal stability and inactivation of hepatitis C virus grown in cell cultureMTT assay of cytotoxicity associated with various concentrations of aldehydes or detergents. Solutions of formaldehyde (37% or glutaraldehyde (50%) (A), SDS (0.1%) (B), and Triton X-100 (0.2%) or NP-40 (0.2%) (C) were diluted serially in cell culture medium by dilution factors indicated in the X-axes, then added to Huh7-25-CD81 cells seeded in 96-well plates. The treated cells were refed with 100 μl of fresh medium after 6 h of incubation. MTT assay was carried out after an additional 72 h as described in Methods. Data are presented as the percentage of cell viability relative to the untreated controls (mean ± SD, n = 3).Back to article page