Skip to main content
Figure 2 | Virology Journal

Figure 2

From: Thermal stability and inactivation of hepatitis C virus grown in cell culture

Figure 2

Inactivation of HCVcc by heat treatment. Aliquots (100 μl) of an HCVcc virus stock (2.5 × 104 FFU/ml) or HCVcc diluted in normal human serum (1.0 × 105 FFU/ml) were incubated in water baths at 56°C (A and C), 60°C and 65°C (B and D). At indicated time points post incubation, samples were removed, cooled on ice-water bath, and their residual infectivity titrated on Huh7-25-CD81 cells. The dashed line indicates the detection limit of the FFU assay (10 FFU/ml). The asterisks denote time points when infectivity was eliminated completely (tested negative in FFU assay and remained negative for HCV NS3 expression to the third cell passage). The decay rate of HCVcc infectivity at 56°C was calculated as 0.089-log/min and 0.119-log/min, for HCVcc in culture medium (A) and human serum (C), respectively, after the data were fit to a linear regression model (R2 = 0.9883 and 0.9813, respectively).

Back to article page