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Table 1 Monoclonal antibody characterisation.

From: Characterisation of a GII-4 norovirus variant-specific surface-exposed site involved in antibody binding

Monoclonal Antibody

Homologous Antigen

Isotype

Titre1

% Reduction in Binding to Urea-Treated Homologous Antigen

mAbGII4v0.5

GII-4v0

IgG1

100

71.8%

mAbGII4v0.8

GII-4v0

IgG2a

10 000

80.9%

mAbGII4v0.10

GII-4v0

IgG2b

10 000

79.8%

mAbGII4v2.5

GII-4v2

IgG1

10 000

44.0%

mAbGII4v2.6

GII-4v2

IgG1

10 000

51.2%

  1. 1Titres were determined by serial dilution between 1:10 and 1:10 000 000 in an EIA. Titres were taken as the reciprocal of the last dilution to give an optical density >0.5 at 450 nm.
  2. Three anti--GII-4v0 mAbs (mAbGII4v0.5, mAbGII4v0.8, mAbGII4v0.10) and two anti-GII-4v2 mAbs (mAbGII4v2.5, mAbGII4v2.6) were characterised for reactivity, isotype and titre. The ability of the five mAbs to recognise their respective homologous antigen that had been chemically denatured was also assessed by ELISA.