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Figure 3 | Virology Journal

Figure 3

From: Characterisation of a GII-4 norovirus variant-specific surface-exposed site involved in antibody binding

Figure 3

Average percent reduction in binding of (a) anti-GII-4v0 and (b) anti-GII-4v2 mAbs to wild-type and mutant VLPs in a cross adsorption ELISA. As described in the Materials and Methods, each mAb was pre-incubated in a blocking step with the antigen indicated on the x-axis, before being transferred to a microtitre plate coated with antigen homologous to the mAbs being tested. Percent reduction in binding was then calculated using a PBS control. Cross absorption assays were repeated 3 times independently and the average data is presented here with bars showing the standard error of the mean. Cartoons representing the antigenic structure of the antigen (as described in Figure 2) are shown above the bars (with corresponding labels below the bars). All mAbs were used at 1:10 000 dilution, except mAbGII4v0.5, which was used at 1:1000.

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