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Table 3 Results from BestKeeper analysis, Bestkeeper vs. Reference gene candidate

From: Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections

Coeff. of corr. [r] (p-Value)

RPII

Act

β2M

L13

PLA

TBP

GAP

PPI

G6P

Tub

CMV

0.75

0.79

0.76

0.13

0.89

0.10

0.92

0.91

0.75

0.95

 

(0.005)

(0.002)

(0.005)

(0.698)

(0.001)

(0.763)

(0.001)

(0.001)

(0.005)

(0.001)

HHV-6

0.79

0.73

0.54

0.30

0.93

0.79

0.94

0.75

0.82

0.97

 

(0.002)

(0.007)

(0.069)

(0.350)

(0.001)

(0.002)

(0.001)

(0.005)

(0.001)

(0.001)

CAMP

0.91

0.18

0.98

0.95

0.99

0.78

0.63

0.99

0.45

0.59

 

(0.002)

(0.662)

(0.001)

(0.001)

(0.001)

(0.022)

(0.092)

(0.001)

(0.268)

(0.127)

SARS

0.48

0.77

0.41

0.27

0.85

0.88

0.46

0.36

0.73

0.84

 

(0.162)

(0.010)

(0.236)

(0.452)

(0.002)

(0.001)

(0.177)

(0.307)

(0.017)

(0.002)

YF

0.90

0.91

0.96

0.25

0.98

0.94

0.99

0.92

0.93

0.92

 

(0.001)

(0.001)

(0.001)

(0.492)

(0.001)

(0.001)

(0.001)

(0.001)

(0.001)

(0.001)

  1. Abbreviations: SD [± CT]: the standard deviation of the CT; BK: BestKeeper; SumV: Sum of viral infection SD values; SumRGC: Sum of reference gene SD values