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Table 1 Cell culture conditions and results of virus kinetics

From: Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections

 

CMV

HHV-6

CAMP

SARS

YF

cell line

MRC-5

CCRF-HSB-2

HepG2

Huh-7D12

HepG2

multiplicity of infection

2.0

0.5

0.5

1.0

0.5

time to maximal infection /h

72

120

24

72

96

max. infected cells %

100

>70

>90

>70

>80

measuring point /h

0,6,12,24,48,72

0,24,48,72,96,120

0,1,3,6,12,24

0,2,4,22,42

0,24,48,72,96