Table 2 Primers for quantification of mRNA expression
Primer name | Primer Sequences 3’ to 5’ | Annealing temperature(°C) | Purpose | Product length |
|---|---|---|---|---|
18SrRNA | F-GACGTGACTGCTCGGTGC R-AACTCGACCGAGGGCACAAG | 62 | Housekeeping | 144bp |
RelA qPCR | F-AGTACCCTGAGGCTATAACTCGC R-TCCGCAATGGAGGAGAAGTC | 62 | qPCR | 133bp |