Fig. 11

Illustrates the expression levels of recombinant proteins and their specific reactions with monoclonal antibodies, specifically VP1VP2 and VP2 alone, in Sf9 cells that were infected with recBacmid BPV: VP2 and recBacmid BPV: VP1VP2 at different time points. In all the panels the first in the left column represents the mock (cell only) as negative control. a At 24-, 48-, and 72-hours post-transfection, the first panel displays the cells infected only with recBacmid BPVVP2 without His-tag, together with the level of expressed VP2 recombinant protein identified with mAb. b The second panel used anti-DE-loop rabbit monoclonal antibodies to identify His-tagged and His-Tag free VP1VP2 and VP2 protein expression levels. c The third panel displayed the expression levels of proteins from His-tagged and His-Tag free VP1VP2 and VP2 that were detected with anti-anti-His-Tag mouse monoclonal antibodies. These proteins were His-tagged and His-Tag free. In this experiment, RFP (red fluorescence antibodies) Alexa-Fluor®-594-conjugated anti-rabbit IgG and antimouse secondary antibodies were used. Generally, the reaction in the fluorescence microscope clearly shows that the rec-proteins from different designs vary in expression at different time points, and the mAbs are highly specific in the detection of the target proteins